https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47541 Wed 24 Jan 2024 14:39:30 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:16223 Wed 11 Apr 2018 17:00:47 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:6933 Wed 11 Apr 2018 15:29:30 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:16779 Wed 11 Apr 2018 11:59:41 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32927 Tue 14 Aug 2018 14:59:36 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:44256 Glycine max) seed is primarily composed of a mature embryo that provides a major source of protein and oil for humans and other animals. Early in development, the tiny embryos grow rapidly and acquire large quantities of sugars from the liquid endosperm of developing seeds. An insufficient supply of nutrients from the endosperm to the embryo results in severe seed abortion and yield reduction. Hence, an understanding of the molecular basis and regulation of assimilate partitioning involved in early embryo development is important for improving soybean seed yield and quality. Here, we used expression profiling analysis to show that two paralogous sugar transporter genes from the SWEET (Sugars Will Eventually be Exported Transporter) family, GmSWEET15a and GmSWEET15b, were highly expressed in developing soybean seeds. In situ hybridization and quantitative real-time PCR showed that both genes were mainly expressed in the endosperm at the cotyledon stage. GmSWEET15b showed both efflux and influx activities for sucrose in Xenopus oocytes. In Arabidopsis (Arabidopsis thaliana), knockout of three AtSWEET alleles is required to see a defective, but not lethal, embryo phenotype, whereas knockout of both GmSWEET15 genes in soybean caused retarded embryo development and endosperm persistence, resulting in severe seed abortion. In addition, the embryo sugar content of the soybean knockout mutants was greatly reduced. These results demonstrate that the plasma membrane sugar transporter, GmSWEET15, is essential for embryo development in soybean by mediating Suc export from the endosperm to the embryo early in seed development.]]> Tue 11 Oct 2022 12:42:46 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:530 Thu 25 Jul 2013 09:10:31 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1116 Sat 24 Mar 2018 08:32:07 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:10167 Sat 24 Mar 2018 08:12:27 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11116 Sat 24 Mar 2018 08:12:26 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20714 Sat 24 Mar 2018 08:06:21 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19081 Sat 24 Mar 2018 08:05:20 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19630 Sat 24 Mar 2018 07:58:15 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30601 −1 (fast freeze) or −6.0°C min⁻¹ (slow freeze). Post-thaw survival was highest with a combination of 0.2 M sucrose and 0.68 M glycerol and when these cryoprotectants were washed out with BWWCAP, regardless of whether spermatozoa were frozen using a fast (motility 14.2 ± 4.7%; PI 20.7 ± 2.0%) or slow (motility 12.0 ± 2.7%; PI 22 ± 4%) cryopreservation rate.]]> Sat 24 Mar 2018 07:28:28 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:21684 Mon 23 Nov 2015 12:25:08 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48396 Mon 22 Apr 2024 14:54:52 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46875 4)₂SO₄ and (NH₄)₂HPO₄ as well as temperature is examined on the hydrothermal carbonization of glucose, fructose and sucrose. Increasing the temperature from 160 to 220 °C increased the yield of hydrothermal carbon for each saccharide for the (NH₄)₂SO₄ solution, whereas (NH₄)₂HPO₄ produced a yield that was independent of temperature. The addition of (NH₄)₂SO₄ increased the yield obtained at 220 °C by 4.27, 7.03 and 2.01 wt% for glucose, fructose and sucrose over the baseline salt free solution, respectively. (NH₄)₂SO₄ also increased the quantity of acid produced and the average size of the hydrothermal carbon spheres. Conversely, (NH₄)₂HPO₄ produced carbon structures consisting of interlocked spherical shapes and produced almost no acidic products. XPS analysis revealed that (NH₄)₂SO₄ incorporated nitrogen and sulfur into the hydrothermal structure, while (NH₄)₂HPO₄ only allowed nitrogen to be incorporated. It was assessed that NH₄⁺ enhances the production of hydrothermal carbon, except in the presence of PO₄³⁻, which prevents the reaction from effectively forming hydrothermal carbon and organic acids.]]> Mon 05 Dec 2022 09:19:32 AEDT ]]>